A comparative study of HPLC-DAD and UPLC-UV methods for simultaneous determination of 11 polyphenols in Moringa oleifera leaves

Purpose: To develop, validate and compare two chromatographic methods - high performance liquid chromatography with diode array detector ((HPLC-DAD) ultraviolet detection (UPLC-UV) for the effective analysis of polyphenols in Moringa oleifera leaves.Methods: HPLC-DAD UPLC-UV were applied accurate determination eleven major leaves. The conditions was determined on C18 column by gradient elution 0.5 % phosphoric acid solution -acetonitrile as eluate, at a flow rate 1.0 mL/min methods, respectively. Detector parameter fixed 203 nm. assay validated systematically.Results: instrumental (HPLC-DAD UPLC-UV) had good linearity, precision,repeatability recovery. For both quantification limits (0.057 0.363 μg/mL) lower than those (0.094 1.532 μg/mL). UPLC method shorter running time more sensitive comparing to HPLC method. After optimization evaluation, baseline 11 compounds separated effectively within 68 34 min, respectively.Conclusion: developed assays successfully utilized thesimultaneous can readily be quality control tools leaves China, showing better separation, organic solvent usage analytical period.